Search results for "CAT A SEQUENCE"

showing 1 items of 1 documents

Molecular analysis of the catechol-degrading bacterial community in a coal wasteland heavily contaminated with PAHs

2010

International audience; A PCR-based molecular tool was developed to estimate the diversity of the catechol-degrading bacterial community in a coal wasteland heavily contaminated with PAHS. A degenerate primer pair specific to catA sequences was designed by multiple alignment of known sequences coding a key intermediate of the β-ketoadiapate pathway degrading catechol, namely catechol 1,2-dioxygenase. The specificity of this primer pair was assessed in 21 pure strains by PCR and sequencing. Comparison of the 16S rDNA and catA phylogenies revealed an absence of congruence between these two genes. The primer set was able to amplify catA sequences in DNA extracts from an industrial soil highly …

DNA BacterialEnvironmental Engineering[SDV]Life Sciences [q-bio]Health Toxicology and MutagenesisCatecholsIndustrial WasteBACTERIAL COMMUNITYActinobacteriaSOIL DNA03 medical and health sciencesPhylogeneticsCATHECOLProteobacteriaBotanySoil PollutantsEnvironmental ChemistryPolycyclic Aromatic HydrocarbonsWaste Management and Disposal030304 developmental biology0303 health sciencesMultiple sequence alignmentBacteriabiologyPhylogenetic tree030306 microbiologybiology.organism_classification16S ribosomal RNAPollutionActinobacteriaBiodegradation EnvironmentalCoalPCR[SDE]Environmental SciencesHorizontal gene transferBIODIVERSITYRestriction fragment length polymorphismPrimer (molecular biology)CAT A SEQUENCEJournal of Hazardous Materials
researchProduct